Part:BBa_K1810003
Optimized Melittin
Melittin is an antimicrobial peptide found in the venom of Apis mellifera. It works by aggregating in the membrane of a bacteria cell and forming a pore, ultimately lysing the cell. An original sequence for melittin in the registry can be found in the BBa_K1382000 BioBrick; however, this sequence was taken directly from Apis Mellifera. In this biobrick, the sequence for melittin has been optimized for E. coli.
While this biobrick for melittin is simply the coding region without a promoter or ribosome binding site, the optimization of the sequence of melittin in comparison to the natural version was confirmed by a construct which fused the melittin sequence with the fluorescent mCherry protein. As the following graph depicts, the optimization of the sequence for melittin resulted in an approximately 100 fold increase in production, as measured in relative fluorescence levels; thus, this sequence represents a significant advancement on the original melittin biobrick (BBa_K1382000).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 42
Illegal AgeI site found at 34 - 1000COMPATIBLE WITH RFC[1000]
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